PUBLICATIONS ｜ Takebe.Lab

High-Fidelity Drug-Induced Liver Injury Screen Using Human Pluripotent Stem Cell–Derived Organoids

Gastroenterology | 2021 February 01

We established a liver organoid-based toxicity screening (LoT) platform using human iPSC-derived organoids. This system enables predictive drug-induced liver injury (DILI) analysis with high sensitivity and specificity, providing insights into bile acid transport, hepatotoxicity mechanisms, and genomic predispositions, advancing drug development and precision medicine.

Tadahiro Shinozawa, Masaki Kimura, Yuqi Cai, Norikazu Saiki, Yosuke Yoneyama, Rie Ouchi, Hiroyuki Koike, Mari Maezawa, Ran-Ran Zhang, Andrew Dunn, Autumn Ferguson, Shodai Togo, Kyle Lewis, Wendy L Thompson, Akihiro Asai, Takanori Takebe

Polygenic architecture informs potential vulnerability to drug-induced liver injury

Nature Medicine | 2020 September 07

Koido et al. revealed genetically defined hepatocyte vulnerability mechanisms to currently unpredictable drug induced liver injury supported by cell-, organoid- and patient-level datasets.

Masaru Koido, Eri Kawakami, Junko Fukumura, Yui Noguchi, Momoko Ohori, Yasunori Nio, Paola Nicoletti, Guruprasad P Aithal, Ann K Daly, Paul B Watkins, Hisashi Anayama, Yvonne Dragan, Tadahiro Shinozawa, Takanori Takebe

Deriving human intestinal organoids with functional tissue-resident macrophages all from pluripotent stem cells

Cellular and Molecular Gastroenterology and Hepatology | 2024 December 17

We developed a human intestinal organoid model incorporating functional tissue-resident macrophages, enabling long-term studies of immune responses and inflammatory mechanisms. This system mimics fetal intestinal macrophages and offers a valuable tool for investigating inflammatory bowel disease.

Kentaro Tominaga, Daniel O Kechele, J Guillermo Sanchez, Simon Vales, Ingrid Jurickova, Lizza Roman, Akihiro Asai, Jacob R Enriquez, Heather A McCauley, Keishi Kishimoto, Kentaro Iwasawa, Akaljot Singh, Yuko Horio, Jorge O Múnera, Takanori Takebe, Aaron M Zorn, Michael A Helmrath, Lee A Denson, James M Wells.

Human pluripotent stem cell-derived organoids repair damaged bowel in vivo

Cell Stem Cell | 2024 October 03

We show that human pluripotent stem cell-derived intestinal organoids repair damaged bowel in a preclinical model. Multi-lineage organoids engraft long-term, forming mesenchymal and epithelial layers, restoring tissue function, and highlighting their therapeutic potential for intestinal injury repair.

Holly M Poling, Nambirajan Sundaram, Garrett W Fisher, Akaljot Singh, Joseph R Shiley, Kalpana Nattamai, Vinothini Govindarajah, Alexander R Cortez, Maksym O Krutko, Séverine Ménoret, Ignacio Anegon, Magdalena Kasendra, James M Wells, Christopher N Mayhew, Takanori Takebe, Maxime M Mahe, Michael A Helmrath.

Self-Assembled Generation of Multi-zonal Liver Organoids from Human Pluripotent Stem Cells

bioRxiv | 2024 August 30

We developed self-assembling, zonally patterned liver organoids from human iPSC-derived hepatic progenitors enriched with ascorbate and bilirubin. These organoids mimic human liver zonation, enabling studies of liver function, development, and disease, and improved survival in bile duct-ligated rats, offering a promising model for therapeutic applications.

Hasan Al Reza, Connie Santangelo, Abid Al Reza, Kentaro Iwasawa, Sachiko Sachiko, Kathryn Glaser, Alexander Bondoc, Jonathan Merola, Takanori Takebe

Eicosatetraynoic Acid Regulates Profibrotic Pathways in an Induced Pluripotent Stem Cell–Derived Macrophage-Human Intestinal Organoid Model of Crohn’s Disease

Journal of Crohn's and Colitis | 2024 August 30

We previously identified small molecules predicted to reverse an ileal gene signature for future Crohn’s Disease (CD) strictures. Here we used a new human intestinal organoid (HIO) model system containing macrophages to test a lead candidate, eicosatetraynoic acid (ETYA).

Ingrid Jurickova, Benjamin W Dreskin, Elizabeth Angerman, Erin Bonkowski, Jack Nguyen, Richard Villarreal, Kentaro Tominaga, Kentaro Iwasawa, Tzipi Braun, Takanori Takebe, Michael A Helmrath, Yael Haberman, James M Wells, Lee A Denson.

ACMSD inhibition corrects fibrosis, inflammation, and DNA damage in MASLD/MASH

Journal of Hepatology | 2024 August 22

We demonstrate that ACMSD inhibition enhances NAD+ synthesis, promotes genome stability, and reduces DNA damage in hepatocytes, effectively preventing MASLD/MASH progression. These therapeutic effects are validated using human liver organoid models, with Mendelian randomization supporting the causal role of DNA damage in MASLD/MASH.

Yasmine J Liu, Masaki Kimura, Xiaoxu Li, Jonathan Sulc, Qi Wang, Sandra Rodríguez-López, Angelique ML Scantlebery, Keno Strotjohann, Hector Gallart-Ayala, Archana Vijayakumar, Robert P Myers, Julijana Ivanisevic, Riekelt H Houtkooper, G Mani Subramanian, Takanori Takebe, Johan Auwerx.

A pillar and perfusion plate platform for robust human organoid culture and analysis

Advanced Healthcare Materials | 2024 August 21

We present a microarray 3D bioprinting technology with pillar and perfusion plates that streamline organoid culture for high-throughput screening. This approach enables scalable stem cell printing, liver and intestine organoid development, and compatibility with standard HTS tools, advancing drug discovery efforts.

Soo‐Yeon Kang, Masaki Kimura, Sunil Shrestha, Phillip Lewis, Sangjoon Lee, Yuqi Cai, Pranav Joshi, Prabha Acharya, Jiafeng Liu, Yong Yang, J Guillermo Sanchez, Sriramya Ayyagari, Eben Alsberg, James M Wells, Takanori Takebe, Moo‐Yeal Lee

Three-dimensional matrix stiffness modulates mechanosensitive and phenotypic alterations in oral squamous cell carcinoma spheroids

APL bioengineering | 2024 July 31

We reveal that oral squamous cell carcinoma spheroids sense extracellular matrix stiffness through mechanosensitive ion channels Piezo1 and TRPV4. Increased stiffness promotes spheroid growth, stemness, and pro-tumorigenic phenotypes, highlighting the role of mechanotransduction in cancer progression.

Maulee Sheth, Manju Sharma, Maria Lehn, HasanAl Reza, Takanori Takebe, Vinita Takiar, Trisha Wise-Draper, Leyla Esfandiari.

Self-Organization of Sinusoidal Vessels in Pluripotent Stem Cell-derived Human Liver Bud Organoids

bioRxiv | 2024 July 24

We directly differentiated human pluripotent stem cells into liver sinusoidal progenitors, generating vascularized liver organoids with functional sinusoid-like vessels. These organoids correct clotting deficiencies in vitro and in hemophilia A models, providing insights into vessel development and therapeutic applications for coagulation disorders.

Norikazu Saiki, Yasunori Nio, Yosuke Yoneyama, Shuntaro Kawamura, Kentaro Iwasawa, Eri Kawakami, Kohei Araki, Junko Fukumura, Tsuyoshi Sakairi, Tamaki Kono, Rio Ohmura, Masaru Koido, Masaaki Funata, Wendy L Thompson, Pamela Cruz-Encarnacion, Ya-Wen Chen, Takanori Takebe.

Inter-cellular mRNA Transfer Alters Human Pluripotent Stem Cell State

| 2024 June 27

We uncover an inter-cellular mRNA transfer mechanism enabling the reprogramming of human primed pluripotent stem cells into a naïve-like state through direct coculture with mouse embryonic stem cells. This phenomenon highlights mRNA mobility’s role in cellular communication and adaptation across species.

Yosuke Yoneyama, Ran-Ran Zhang, Masaki Kimura, Yuqi Cai, Mike Adam, Sreeja Parameswaran, Hideki Masaki, Naoaki Mizuno, Joydeep Bhadury, So Maezawa, Hiroshi Ochiai, Hiromitsu Nakauchi, S Steven Potter, Matthew T Weirauch, Takanori Takebe.

Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities

Cells | 2024 January 11

We identified a subpopulation of CD29+ bovine muscle progenitor cells with high adipogenic potential for cultured meat production. By isolating CD29+CD44−CD344− cells, we developed stable, fat-rich “meat buds,” paving the way for improved quality control and reliable stem cell-based meat production techniques.

Yuna Naraoka, Yo Mabuchi, Mai Kiuchi, Kyoko Kumagai, Daisuke Hisamatsu, Yosuke Yoneyama, Takanori Takebe, Chihiro Akazawa

Self-organized yolk sac-like organoids allow for scalable generation of multipotent hematopoietic progenitor cells from induced pluripotent stem cells

Cell | 2023 October 27

We report a production protocol that overcomes these hurdles and that allows for mass production of HPCs from hiPSCs via the induction of yolk sac-like organoids without addition of exogenous factors.

Tamaoki N, Siebert S, Maeda T, Ha NH, Good ML, Huang Y, Vodnala SK, Haro-Mora JJ, Uchida N, Tisdale JF, Sweeney CL, Choi U, Brault J, Koontz S, Malech HL, Yamazaki Y, Isonaka R, Goldstein DS, Kimura M, Takebe T, Zou J, Stroncek DF, Robey PG, Kruhlak MJ, Restifo NP, Vizcardo R

Complement factor D targeting protects endotheliopathy in organoid and monkey models of COVID-19

Cell Stem Cell | 2023 October 05

We developed an infection-competent human vascular organoid from pluripotent stem cells for modeling endotheliopathy. Longitudinal serum proteome analysis identified aberrant complement signature in critically ill patients driven by the amplification cycle regulated by complement factor B and D (CFD).

Kawakami E, Saiki N, Yoneyama Y, Takayama K, Nio Y, Takebe T

Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells

STAR protocols | 2023 July 28

We present a protocol for preparing hydrogels with mechanical patterns. We describe steps for hydrogel synthesis, mechanical evaluation of the substrate, and time-lapse imaging of cell self-organization.

Matsuzaki T, Kawano Y, Horikiri M, Shimokawa Y, Yamazaki T, Okuma N, Koike H, Kimura M, Kawamura R, Yoneyama Y, Furuichi Y, Hakuno F, Takahashi SI, Nakabayashi S, Okamoto S, Nakauchi H, Taniguchi H, Takebe T, Yoshikawa HY.

En masse organoid phenotyping informs metabolic-associated genetic susceptibility to NASH

Cell | 2022 October 27

We devised a pooled human organoid-panel of steatohepatitis to investigate the impact of metabolic status on genotype-phenotype association.

Kimura M, Iguchi T, Iwasawa K, Dunn A, Thompson W, Yoneyama Y, Chaturvedi P, Zorn A, Wintzinger M, Quattrocelli M, Watanabe-Chailland M, Zhu G, Fujimoto M, Kumbaji M, Kodaka A, Gindin Y, Chung C, Myers R, Subramanian G, Hwa V, Takebe T

Watch the video abstract

Mechanical guidance of self-condensation patterns of differentiating progeny

iScience | 2022 September 27

We have developed a mechanically patterned hydrogel for controlling self-condensation process to generate multi-cellular organoids.

Matsuzaki T, Shimokawa Y, Koike H, Kimura M, Kawano Y, Okuma N, Kawamura R, Yoneyama Y, Furuichi Y, Hakuno F, Takahashi S, Nakabayashi S, Okamoto S, Nakauchi H, Taniguchi H, Takebe T, Yoshikawa H

Cell membrane fluidity and ROS resistance define DMSO tolerance of cryopreserved synovial MSCs and HUVECs

Stem cell research & therapy | 2022 May 03

This study aimed to identify the biological and physical factors involved in the differences in freeze–thaw tolerance between MSCs and HUVECs.

Mizuno M, Matsuzaki T, Ozeki N, Katano H, Koga H, Takebe T, Yoshikawa HY & Sekiya I

Eicosatetraynoic Acid and Butyrate Regulate Human Intestinal Organoid Mitochondrial and Extracellular Matrix Pathways Implicated in Crohn’s Disease Strictures

Inflammatory Bowel Diseases | 2022 March 08

We developed a patient-specific human intestinal organoid (HIO) model system to test small molecule regulation of mitochondrial and wound-healing functions implicated in stricturing behavior.

Ingrid Jurickova et al

Enteral liquid ventilation oxygenates a hypoxic pig model

iScience | 2022 February 13

We assessed the enteral ventilation approach in hypoxic porcine models under controlled mechanical ventilation.

Fujii T, Yoneyama Y, Kinebuchi A, Ozeki N, Maeda S, Saiki N, Fengshi Chen-Yoshikawa T.F., Date H, Nishiwaki K, Takebe T

Correction of a Factor VIII genomic inversion with designer-recombinases

Nature Communications | 2022 January 20

In this work, Lansing et al. present designer-recombinases as an efficient and specific means towards treatment of monogenic diseases caused by large gene inversions.

Lansing F, Mukhametzyanova L, Rojo-Romanos T. et al.

Synthesis and application of POLYseq for profiling human liver organoids

STAR Protocols | 2021 December 24

Dunn et al. updated the protocol for POLYseq using inexpensive, commercially available reagents.

Dunn A, Cai Y, Iwasawa K, Kimura M, Takebe T

Isolation and Characterization of Tissue Resident CD29-Positive Progenitor Cells in Livestock to Generate a Three-Dimensional Meat

Cells | 2021 September 21

Naraoka et al. screened 246 cell-surface antibodies by fluorescence-activated cell sorting for their capacity to form colonies and their suitability to construct spheroid “meat buds”.

Naraoka Y, Mabuchi Y, Yoneyama Y, Suto EG, Hisamatsu D, Ikeda M, Ito R, Nakamura T, Takebe T, Akazawa C

POLYseq: A poly(β-amino ester)-based vector for multifunctional cellular barcoding

Stem Cell Reports | 2021 August 26

Dunn et al. developed a novel poly(β-amino) ester labeling system synthesized with inexpensive, common reagents, termed POLYseq, capable of efficiently delivering fluorescent molecules or sample-distinguishing DNA barcodes through non-covalent binding enabling rapid creation of custom sample pools.

Andrew Dunn, Yuqi Cai, Kentaro Iwasawa, Masaki Kimura, Takanori Takebe.

Mammalian enteral ventilation ameliorates respiratory failure

Med | 2021 May 14

Okabe el al. showed the effectiveness of an enteral ventilation approach in attaining systemic oxygenation in both rodent and porcine models, inspired by organisms such as loaches that use intestinal air breathing.

Ryo Okabe, Toyofumi F. Chen-Yoshikawa, Yosuke Yoneyama. Eiji Kobayashi, Hiroshi Date, Takanori Takebe.

Selected as Cover Article

Highlighted in Med Viewpoint, NYTimes, Economist, Science, Smithsonian Magazine, NYPost, National Geographic, TrontoStar the others.

Watch the video

Common Genetic Variation in Humans Impacts In Vitro Susceptibility to SARS-CoV-2 Infection

Stem Cell Reports | 2021 March 09

Dobrindt et al. applied human induced pluripotent stem cell (hiPSC)-based models and CRISPR engineering to explore the host genetics of SARS-CoV-2.

Dobrindt K, Hoagland DA, Seah C, Kassim B, O’Shea CP, Murphy A, Iskhakova M, Fernando MB, Powell SK, Deans PJM, Javidfar B, Peter C, Møller R, Uhl SA, Garcia MF, Kimura M, Iwasawa K, Crary JF, Kotton DN, Takebe T, Huckins LM, tenOever BR, Akbarian S, Brennand KJ

Modeling Human Bile Acid Transport and Synthesis in Stem Cell-Derived Hepatocytes with a Patient-Specific Mutation

Stem Cell Reports | 2021 February 09

Hayashi el al. developed a human induced pluripotent stem cell-based model of isogenic BSEP-deficient hepatocytes in a Transwell culture system.

Hayashi H, Osaka S, Sakabe K, Fukami A, Kishimoto E, Aihara E, Sabu Y, Mizutani A, Kusuhara H, Naritaka N, Zhang W, Huppert SS, Sakabe M, Nakamura T, Hu YC, Mayhew C, Setchell K, Takebe T, Asai A

High-Fidelity Drug-Induced Liver Injury Screen Using Human Pluripotent Stem Cell–Derived Organoids

Gastroenterology | 2021 February 01

We established a liver organoid-based toxicity screening (LoT) platform using human iPSC-derived organoids. This system enables predictive drug-induced liver injury (DILI) analysis with high sensitivity and specificity, providing insights into bile acid transport, hepatotoxicity mechanisms, and genomic predispositions, advancing drug development and precision medicine.

Tadahiro Shinozawa, Masaki Kimura, Yuqi Cai, Norikazu Saiki, Yosuke Yoneyama, Rie Ouchi, Hiroyuki Koike, Mari Maezawa, Ran-Ran Zhang, Andrew Dunn, Autumn Ferguson, Shodai Togo, Kyle Lewis, Wendy L Thompson, Akihiro Asai, Takanori Takebe

Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells

Nature Protocol | 2021 January 11

Koike et al. describe a protocol for the continuous patterning of hepatic, biliary and pancreatic (HBP) structures from a 3D culture of human pluripotent stem cells (PSCs).

Koike H, Iwasawa K, Ouchi R, Maezawa M, Kimura M, Kodaka A, Nishii S, Thompson WL, Takebe T

Simultaneous Zn2+ tracking in multiple organelles using super-resolution morphology-correlated organelle identification in living cells

Nature Communications | 2021 January 04

Fang et al. developed a new turn-on Zn2+ fluorescent probe, NapBu-BPEA, to ensure SIM imaging quality for organelle identification.

Fang H, Geng S, Hao M, Chen Q, Liu M, Liu C, Tian Z, Wang C, Takebe T, Guan JL, Chen Y, Guo Z, He W, Diao J

Self-organized yolk sac-like organoids allow for scalable generation of multipotent hematopoietic progenitor cells from human induced pluripotent stem cells

bioRxiv | 2021 January 01

Tamaoki et al. showed that hiPSCs co-cultured with various types of stromal cells as spheroids self-organize into yolk sac-like organoids without the addition of exogenous factors.

Tamaoki N, Siebert S, Maeda T, Ngoc-H Ha, Good M L, Huang Y, Vodnala S K, Haro-Mora J J, Uchida N, Tisdale J F, Sweeney C L, Choi U, Brault J, Koontz S, Malech H L, Yamazaki Y, Isonaka R, Goldstein D S, Kimura M, Takebe T, Zou J, Stroncek D F, Robey P G, Kruhlak M J, Restifo N P, Vizcardo R

Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media

Scientific reports | 2020 October 21

CD-AOF media and supplements ensure the quality and reproducibility of culture systems. This protocol will facilitate the clinical applicability of all iPSC-LBs in the treatment of liver diseases.

Sekine K, Ogawa S, Tsuzuki S, Kobayashi T, Ikeda K, Nakanishi N, Takeuchi K, Kanai E, Otake Y, Okamoto S, Kobayashi T, Takebe T, Taniguchi H

Stiffness distribution analysis in indentation depth direction reveals clear mechanical features of cells and organoids by using AFM

Applied Physics Express | 2020 August 27

Kobayashi et al. proposed a method to statistically analyze stiffness distribution in indentation depth direction from multiple force curves measured by AFM.

Kobayashi N, Togo S, Matsuzaki T, Hashiseko K, Kawamura R, Suganuma M, Nakabayashi S, Yoneyama Y, Ouchi R, Takebe T, Yoshikawa H

Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis

Nature Communications | 2020 August 27

Han et al. identified unexpected diversity in mesoderm-derivatives during early foregut organogenesis. This study is highly collaborative work with Aaron Zorn group and Mitsuru Morimoto group at RIKEN.

Lu Han, Praneet Chaturvedi, Keishi Kishimoto, Hiroyuki Koike, Talia Nasr, Kentaro Iwasawa, Kirsten Giesbrecht, Phillip C Witcher, Alexandra Eicher, Lauren Haines, Yarim Lee, John M Shannon, Mitsuru Morimoto, James M Wells, Takanori Takebe, Aaron M Zorn

Waiting Time Analysis at University Hospitals Based on Visitor Psychology

International Conference on Human-Computer Interaction | 2020 July 19

Shigeyoshi Iizuka, Shozo Nishii, Eriko Tanimoto, Hiro Nakazawa, Asuka Kodaka, Takanori Takebe

Generation of multi-cellular human liver organoids from pluripotent stem cells

Methods in Cell Biology | 2020 May 22

Wendy elaborated a human liver organoid culture protocol based on Ouchi et al. Cell Metabolism paper in 2019.

Wendy L. Thompson, Takanori Takebe

Orthotopic foetal lung tissue direct injection into lung showed a preventive effect against paraquat-induced acute lung injury in mice

European Journal of Cardio-Thoracic Surgery | 2020 April 07

Okabe et al., in collaboration with thoracic surgeons at Kyoto, extended fetal organ bud transplantation approach towards acute lung injury protection in mouse model.

Ryo Okabe, Toyofumi F Chen-Yoshikawa, Akihiko Yoshizawa, Tsuyoshi Hirashima, Masao Saito, Hiroshi Date, Takanori Takebe

The beta-catenin/YAP signaling axis is a key regulator of melanoma-associated fibroblasts

Signal transduction and targeted therapy | 2019 December 24

T Liu, L Zhou, K Yang, K Iwasawa, AL Kadekaro, T Takebe, T Andl, Y Zhang.

De Novo-Designed Near-Infrared Nanoaggregates for Super-Resolution Monitoring of Lysosomes in Cells, in Whole Organoids and In Vivo

ACS Nano | 2019 December 04

Fang et al. designed a low-toxicity, photostable, near-infrared small molecule fluorescence probe HD-Br for use in the super-resolution imaging of lysosomes.

Fang, H., Yao, S., Chen,Q., Liu, C., Cai, Y., Geng, S., Bai, Y., Tian, Z., Zacharias, A. L., Takebe, T., Chen, Y., Guo, Z., He, W., and Diao, J.

Modelling human hepato-biliary-pancreatic organogenesis from the foregut–midgut boundary

Nature | 2019 September 25

Koike et al. established a multi-organ connection in human stem cell derived organoid model amongst liver, biliary, pancreatic and duodenal tissues.

Koike, H., Iwasawa, K., Ouchi, R., Maezawa, M., Giesbrecht, K., Saiki, N., Ferguson A., Kimura, M., Thompson, W., Wells, J. M., Zorn, A. M. & Takebe, T.

Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids

Cell Metabolism | 2019 May 30

Ouchi et al. established a method to generate multicellular organoid models of inflammation and fibrosis that can be used for genetic steatohepatitis modeling.

Ouchi, R., Togo, S., Kimura, M., Shinozawa, T., Koido, M., Koike, H., Thompson, W., Karns, R. A., Mayhew, C. N., McGrath, P. S., McCauley, H. A., Zhang, R. R., Lewis, K., Hakozaki, S., Ferguson, A., Saiki, N., Yoneyama, Y., Takeuchi, I., Mabuchi, Y., Akazawa, C., Yoshikawa, H. Y., Wells, J. M., Takebe, T.*

Featured in Nature Reviews
Featured in NIH Director’s Blog

Defining Lineage-Specific Membrane Fluidity Signatures that Regulate Adhesion Kinetics

Stem Cell Reports | 2018 October 09

Takahisa Matsuzaki, Shinya Matsumoto, Toshiharu Kasai, Hiroshi Y. Yoshikawa, Hideki Taniguchi, Takanori Takebe

Optimal Hypoxia Regulates Human iPSC-Derived Liver Bud Differentiation through Intercellular TGFB Signaling

Stem Cell Reports | 2018 September 01

Hiroaki Ayabe, Takahisa Anada, Takuo Kamoya, Tomoya Sato, Masaki Kimura, Emi Yoshizawa, Shunyuu Kikuchi, Yasuharu Ueno, Keisuke Sekine, Gray Camp, Barbara Treutlein, Autumn Ferguson, Osamu Suzuki, Takanori Takebe, Hideki Taniguchi

Digitalized Human Organoid for Wireless Phenotyping

iScience | 2018 May 31

Masaki Kimura, Momoko Azuma, Ran-Ran Zhang, Wendy Thompson, Christopher N. Mayhew, Takanori Takebe

Self-Condensation Culture Enables Vascularization of Tissue Fragments for Efficient Therapeutic Transplantation

Cell Reports | 2018 May 08

Selected as cover article

Yoshinobu Takahashi, Keisuke Sekine, Tatsuya Kin, Takanori Takebe, Hideki Taniguchi

Human iPSC-Derived Posterior Gut Progenitors Are Expandable and Capable of Forming Gut and Liver Organoids

Stem Cell Reports | 2018 March 13

Ran-Ran Zhang, Masaru Koido, Tomomi Tadokoro, Rie Ouchi, Tatsuya Matsuno, Yasuharu Ueno, Keisuke Sekine, Takanori Takebe, Hideki Taniguchi

Tumoroid à la carte: Path for personalization

Hepatology | 2018 February 19

Kyle Lewis, Takanori Takebe

Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells

Cell Reports | 2017 December 05

Takanori Takebe, Keisuke Sekine, Masaki Kimura, Satoshi Okamoto, Yasuharu Ueno, Hideki Taniguchi

Synergistic Engineering: Organoids Meet Organs-on-a-Chip

Cell Stem Cell | 2017 September 07

Takanori Takebe, Boyang Zhang, Milica Radisic

Multilineage communication regulates human liver bud development from pluripotency

Nature | 2017 June 14

Featured in Science
Featured in Cell Systems

J. Gray Camp, Keisuke Sekine, Tobias Gerber, Henry Loeffler-Wirth, Hans Binder, Malgorzata Gac, Sabina Kanton, Jorge Kageyama, Georg Damm, Daniel Seehofer, Lenka Belicova, Marc Bickle, Rico Barsacchi, Ryo Okuda, Emi Yoshizawa, Masaki Kimura, Hiroaki Ayabe, Hideki Taniguchi, Takanori Takebe & Barbara Treutlein

Advances in Organoid Technology: Hans Clevers, Madeline Lancaster, and Takanori Takebe

Cell Stem Cell, Q&A | 2017 June 01

Hans Clevers, Madeline Lancaster, and Takanori Takebe

Nutritional modulation of mouse and human liver bud growth through a branched-chain amino acid metabolism

Development | 2017 March 14

Hiroyuki Koike, Ran-Ran Zhang, Yasuharu Ueno, Keisuke Sekine, Yun-Wen Zheng, Takanori Takebe, Hideki Taniguchi

Reverse engineering liver buds through self-driven condensation and organization towards medical application

Developmental Biology | 2016 December 15

Tadahiro Shinozawa, Hiroshi Y. Yoshikaw, Takanori Takebe

Growing Mini‐Organs from Stem Cells

Reviews in Cell Biology and Molecular Medicine | 2016 November 01

Hiroyuki Koike, Tamir Rashid, Takanori Takebe

Autotransplantation of Monkey Ear Perichondrium-Derived Progenitor Cells for Cartilage Reconstruction

Cell Transplantation | 2016 May 01

Shintaro Kagimoto, Takanori Takebe, Shinji Kobayashi, Yuichiro Yabuki, Ayaka Hori, Koichi Hirotomi, Taro Mikami, Toshimasa Uemura, Jiro Maegawa, Hideki Taniguchi

Generating Mini-Organs in Culture

Current Pathobiology Reports | 2016 April 11

Hiroyuki Koike & Takanori Takebe

Engineering pancreatic tissues from stem cells towards therapy

Regenerative Therapy | 2016 March 01

Yoshinobu Takahashi, Takanori Takebe, Hideki Taniguchi

The boom in mini stomachs, brains, breasts, kidneys and more

Nature, News Feature | 2015 July 29

Cassandra Willyard

Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell-Driven Condensation

Cell Stem Cell | 2015 May 07

Selected as Cover Article Best of Cell Stem Cell, 2015 News and Views in Cell Stem Cell Featured in Nature Methods

TakanoriTakebe, Masahiro Enomura, Emi Yoshizawa, Masaki Kimura, Hiroyuki Koike, Yasuharu Ueno, Takahisa Matsuzaki, Takashi Yamazaki, Takafumi Toyohara, Kenji Osafune, Hiromitsu Nakauchi, Hiroshi Y.Yoshikawa, Hideki Taniguchi

Transient vascularization of transplanted human adult–derived progenitors promotes self-organizing cartilage

Journal of Clinical Investigation | 2014 September 09

Takanori Takebe, Shinji Kobayashi, Hiromu Suzuki, Mitsuru Mizuno, Yu-Min Chang, Emi Yoshizawa, Masaki Kimura, Ayaka Hori, Jun Asano, Jiro Maegawa, and Hideki Taniguchi

Novel strategies for liver therapy using stem cells

Stem Cells and Development | 2014 September 02

Rashid T, Takebe T, Nakauchi H.

Brief Report: Reconstruction of Joint Hyaline Cartilage by Autologous Progenitor Cells Derived from Ear Elastic Cartilage

STEM CELLS | 2014 August 23

Mitsuru Mizuno, Shinji Kobayashi, Takanori Takebe, Hiroomi Kan, Yuichiro Yabuki, Takahisa Matsuzaki, Hiroshi Y. Yoshikawa, Seiichiro Nakabayashi, Lee Jeong Ik, Jiro Maegawa, Hideki Taniguchi

Generation of pancreatic beta-cells from pluripotent stem cells

Organ Biology | 2014 July 10

Yoshinobu Takahashi, Takanori Takebe, Hideki Taniguchi.

The CD133 + CD44 + Precancerous Subpopulation of Oval Cells Is a Therapeutic Target for Hepatocellular Carcinoma

Stem Cells and Development | 2014 June 26

Yun-Wen Zheng, Tomonori Tsuchida, Taiki Shimao, Bin Li, Takanori Takebe, Ran-Ran Zhang, Yu Sakurai, Yasuharu Ueno, Keisuke Sekine, Naoto Ishibashi, Makiko Imajima, Takuji Tanaka, and Hideki Taniguchi

Human iPSC‐Derived Miniature Organs: A Tool for Drug Studies

Clinical Pharmacology & Therapeutics | 2014 March 21

T Takebe, H Taniguchi.

Engineering of human hepatic tissue with functional vascular networks

Organogenesis | 2014 February 22

Takanori Takebe, Naoto Koike, Keisuke Sekine, Ryoji Fujiwara, Takeru Amiya, Yun-Wen Zheng & Hideki Taniguchi

Ring1B promotes hepatic stem/progenitor cell expansion through simultaneous suppression of Cdkn1a and Cdkn2a in mice

Hepatology | 2014 February 04

Hiroyuki Koike, Yasuharu Ueno, Takako Naito, Tomoya Shiina, Susumu Nakata, Rie Ouchi, Yuta Obana, Keisuke Sekine, Yun‐Wen Zheng, Takanori Takebe, Kyo‐ichi Isono, Haruhiko Koseki, Hideki Taniguchi

Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant

Nature Protocols | 2014 January 23

Takanori Takebe, Ran-Ran Zhang, Hiroyuki Koike, Masaki Kimura, Emi Yoshizawa, Masahiro Enomura, Naoto Koike, Keisuke Sekine & Hideki Taniguchi

Vascularized and functional liver devided from human induced pluripotent stem cells by recapitulating organogenesis

Yokohama Medical Journal | 2014 January 01

T. Takebe, H. Taniguchi.

Creation of vascularized and functional organ from human iPS cell

Organ Biology | 2013 July 10

Takanori Takebe, Hideki Taniguchi.

Vascularized and functional human liver from an iPSC-derived organ bud transplant

Press in BBC, The Economist, Scientific American, The Guardian, etc… | 2013 July 03

Selected as Breakthrough of the Year, 2013 in Science

Takanori Takebe, Keisuke Sekine, Masahiro Enomura, Hiroyuki Koike, Masaki Kimura, Takunori Ogaeri, Ran-Ran Zhang, Yasuharu Ueno, Yun-Wen Zheng, Naoto Koike, Shinsuke Aoyama, Yasuhisa Adachi & Hideki Taniguchi

Selected as World top 5 discovery in Discover magazineNews and Views in NatureFeatured in Science, Nature Medicine, Cell Stem Cell, Hepatology, Gastroenterology, Cell Research, etc…

The generation of pancreatic β-cell spheroids in a simulated microgravity culture system

Biomaterials | 2013 July 01

Hiroyasu Tanaka, Shin Tanaka, Keisuke Sekine, Sayaka Kita, Ai Okamura, Takanori Takebe, Yun-Wen Zheng, Yasuharu Ueno, Junzo Tanaka, Hideki Taniguchi

Establishment of automated culture system for murine induced pluripotent stem cells

BMC Biotechnology | 2012 November 01

Hiroyuki Koike, Koji Kubota, Keisuke Sekine, Takanori Takebe, Rie Ouchi, Yun-Wen Zheng, Yasuharu Ueno, Naoki Tanigawa, Hideki Taniguchi

Generation of Functional Human Vascular Network

Transplantation Proceedings | 2012 July 01

T Takebe, N Koike, K Sekine, M Enomura, Y Chiba, Y Ueno, Y-W Zheng, H Taniguchi

Rudimentary liver grown in vitro

Nature, News Feature | 2012 June 20

David Cyranoski

Self-organization of Human Hepatic Organoid by Recapitulating Organogenesis in Vitro

Transplantation Proceedings | 2012 May 01

T Takebe, K Sekine, Y Suzuki, M Enomura, S Tanaka, Y Ueno, Y-W Zheng, H Taniguchi

Presence of Cartilage Stem/Progenitor Cells in Adult Mice Auricular Perichondrium

PLOS ONE | 2011 October 19

Shinji Kobayashi ,Takanori Takebe ,Yun-Wen Zheng,Mitsuru Mizuno,Yuichiro Yabuki,Jiro Maegawa,Hideki Taniguchi

Reconstruction of human elastic cartilage by a CD44+ CD90+ stem cell in the ear perichondrium

PNAS | 2011 August 30

Shinji Kobayashi, Takanori Takebe, Midori Inui, Sayaka Iwai, Hiroomi Kan, Yun-Wen Zheng, Jiro Maegawa, and Hideki Taniguchi

Reconstruction of human elastic cartilage by a CD44+ CD90+ stem cell in the ear perichondrium

PNAS | 2011 August 30

Shinji Kobayashi, Takanori Takebe, Midori Inui, Sayaka Iwai, Hiroomi Kan, Yun-Wen Zheng, Jiro Maegawa, and Hideki Taniguchi

Synthetic human gonadal tissues for toxicology

Reproductive Toxicology | 2024 May 08

This review highlights 2D and 3D in vitro culture systems, including organoids, as tools for studying human reproductive toxicology and pathology. These models replicate reproductive organ physiology and improve drug screening and toxicity testing by bridging gaps between animal and human studies.

Toshiya Nishimura, Takanori Takebe

Organoid-guided precision hepatology for metabolic liver disease

Journal of Hepatology | 2024 January 17

In this review, Taka and Sho outline current research efforts focused on integrating multiple synthetic tissue models of key metabolic organs, with an emphasis on organoid-based systems.

Sho Osonoi, Takanori Takebe

Narrative engineering of the liver

Current Opinion in Genetics & Development | 2022 August 01

Lee and Takebe discuss advancements in liver organoid technology through the lens of ‘narrative engineering’ in an attempt to synergize evolving understanding around molecular and cellular landscape governing hepatogenesis with engineering-inspired approaches for organoidgenesis in this review.

Inkyu S Lee, Takanori Takebe

Creativity for a cure

Nature Medicine | 2019 June 30

Invited mini-personal story.

Takanori Takebe

Organoids by design

Science | 2019 June 01

Discussed current and future of designing complex organoids.

Takanori Takebe*, James M. Wells

Masaki Kimura, Takanori Takebe.

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